FinePointe 全身体积描记系统-WBP系统

DSI Buxco 全身体积描记系统简称 WBP系统 (Whole Body Plethysmography), 主要用于监测清醒自由活动、非束缚、非麻醉状态下实验动物的呼吸功能。监测过程中，实验动物需放置于特殊设计的腔体中进行实验。WBP 腔体配有高敏感度的压力传感器，可探测腔体内细微的压力变化。腔体上亦配有带筛孔的pneumotachs，通过它，气流可从腔体外进入到腔体内，细微的压力变化由此产生，而腔体本身就是描记仪。基于这些细微的流量信号，Buxco FinePointe 软件通过专业的衍算公式与分析，为您提供专业精准的肺通气、支气管收缩等相关参数。

WBP 系统特性:

适用种属: 小鼠、幼鼠、大鼠、豚鼠、以及其他1千克左右体重的实验动物；大动物可参照大动物WBP系统对照表
可同时监测多达16只动物，有双腔体控制器和四腔体控制器可选，配置灵活
与软件整合的自动化控制偏流仪
预先校准的数字化温湿度信号
标配雾化给药控制器
标配气体自动化功能
可拆卸式控制器设计进一步增强系统配置灵活性 (仅适用于四腔体控制器)
鼠标单击即可实现同时多达4个腔体的全自动校准
校准过程中有全自动化微处理器全程协助诊断
可与其他相关研究整合 - 流体旋转 (fluid swivels）或电子换向器等

WBP 系统优势

自动诊断 – 微处理器全程自动诊断整套系统，确保数据精准度
智能整合技术 – 双向沟通确保科学数据的精准度与再现性
自动校准 – 可一键自动化快速校准，避免手工操作失误
腔体采用 Halcyon 降噪专利技术
雾化给药控制器校准 – 避免了不同控制器给药频率差异
曝露过程中可自动降低流量率损耗
偏流仪降噪技术 – 降低信噪比
Finepointe 软件 – 专业整合多种呼吸相关研究以及自动出报告功能
软件可自动切换多达四通道不同的气体，也可通过外置控制器操作

主要参数详单

参数	参数描述	单位
f	Respiratory Rate	BPM
TVb	Tidal volume (volume inhaled) estimated from the Box Flow signal. It is equal to the product of the volume measured from the Box Flow and Comp	mL
MVb	Minute volume (the rate of ventilation) estimated from the Box Flow signal. It is equal to the product of the volume measured from the Box Flow, f, and Comp	mL/min
Penh	Index of constriction.	None
PAU	Index of constriction.	None
Rpef	The location into expiration where the peak occurs as a fraction of Te	None
Comp	The factor applied to box flow to estimate animal’s flow	None
PIFb	Estimated peak inspiratory flow	mL/s
PEFb	Estimated peak expiratory flow	mL/s
Ti	Inspiratory time	S
Te	Expiratory time	S
EF50	Expiratory flow at 50% expired volume (Uncompensated)	mL/s
EIP	End inspiratory pause. Time it takes to transition from inspiration to expiration	ms
EEP	End expiratory pause. Time it takes to transition from expiration to inspiration	ms
Tr	Relaxation time.	S
TB	Duration of breaking. Percentage of the breath occupied by transitioning from inspiration to expiration	%
TP	Duration of pause before inspiration. Percentage of the breath occupied by transition from expiration to inspiration	%
Tbody	Body temperature. Either assumed or measured using telemetry	C
Tc	Chamber temperature. Either assumed or measured using digital temperature sensor in the chamber	C
RH	Relative humidity. Either assumed or measured using digital humidity sensor in the chamber	%
Rinx	Rejection index. Percentage of breaths rejected before a breath is accepted.	%

参考文献

Publications citing FinePointe whole body plethysmography

Bosnjak B, Tilp C, Tomsic C, Dekan G, Pieper M, Erb K, EpsteinM. “Tiotropium bromide inhibits relapsing allergic asthma in BALB/c mice.” Pulmonary Pharmacology and Therapeutics. 2014;27:44-51.

Haapakoski R, Karisola P, Fyhrquist N, Savinko T, Wolff H, Turjanmaa K, Palosuo T, Reunala T, Lauerma A, Alenius H. “Intradermal cytosine-phosphate-guanosine treatment reduces lung inflammation but induces IFN-gamma-mediated airway hyperreactivity in a murine model of natural rubber latex allergy.” American journal of respiratory cellular and molecular biology. 2011;44:639-647.

Rajavelu P, Chen G, Xu Y, Kitzmiller J, Korfhagen T, Whitsett J. “Airway epithelial SPDEF integrates goblet cell differentiation and pulmonary Th2 inflammation.” Journal of Clinical Investigation. 2015.

Viby N, Isidor M, Buggeskov K, Poulsen S, Hansen J, Kissow K. “Glucagon-Like Peptide-1 (GLP-1) Reduces Mortality and Improves Lung Function in a Model of Experimental Obstructive Lung Disease in Female Mice.” Endocrinology. 2013;154:4503–4511.

Glynn A, Alves D, Frick O, Erwin-Cohen R, Porter A, Norris S, Waag D, Nalca A. “Comparison of experimental respiratory tularemia in threenonhuman primate species.” Comparative Immunology, Microbiology and Infectious Diseases. 2015;39:13-24

WBP 系统主要特性
Invasive	无创式
Restrained	非束缚
Acute	纵向研究
小动物	Large Animal
End Points
频率 / 容积 / 流量
呼吸阻抗
Compliance
Lung Characterization
常见研究领域
支气管哮喘
睡眠呼吸中止
COPD
Fibrosis
Tuberculous
咳嗽
呼吸抑制
安评
整合给药方式
雾化给药
IV
接线给药